Title of article :
The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer Copyright
Author/Authors :
Lakooraj, Hamed Mansoor Department of Clinical Pathology - Faculty of Veterinary Medicine - University of Tehran, Iran , Khaki, Zohreh Department of Clinical Pathology - Faculty of Veterinary Medicine - University of Tehran, Iran , Ghorbani, Masoud Pasteur Institute of Iran - Research and Production Complex - Department of Research and Development , Shafiee Ardestani, Mehdi Department of Radiopharmacy - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Dezfoulian, Omid Department of Pathobiology - Faculty of Veterinary Medicine - Lorestan University, Khorramabad, Iran
Abstract :
BACKGROUND:
To achieve delivery of a drug to tumors, folic acid (FA) was used as a targeting ligand to
change nanocarriers. Since the folate receptor has more expression in several tumor types such as breast cancer.
OBJECTIVES:
The present study aimed to evaluate the effect of Doxorubicine-G2-FA (Dox- G2-FA) with
in vitro assays. The abbreviation of G2 represents the second generation of dendrimer synthesis.
METHODS:
For this purpose, Dox-G2-FA was synthesized and mass spectroscopy was used to confirm the
synthesized component. Also, MTT assay, flow cytometry, and gene expression assay by real-time PCR were
used to evaluate cell viability, apoptosis, and necrosis.
RESULTS:
In this study, the effect of Dox and Dox-G2-FA on the expression of Bax, Bcl2 genes showed
that there was a significant decrease in the expression of the Bcl2 gene in the Dox-G2-FA group compared to
Dox and control groups(
P<0.05). Also, the results of flow-cytometry showed that apoptosis in the presence of
Dox-G2-FA was greater than in the Dox group (
P<0.05).
CONCLUSIONS:
Therefore, it seems that the effect of Dox-G2-FA on apoptosis is better than the effect of
Dox usage alone
Keywords :
Apoptosis , Bax , Bcl2 , Breast cancer , Doxorubicine-G2-folate
Journal title :
Iranian Journal of Veterinary Medicine (IJVM)
