Author/Authors :
Shokoohinia, Yalda Pharmaceutical Sciences Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Bahrami, Gholamreza Medical Biology Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Taherabadi, Fatemeh Pharmaceutical Sciences Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Jaffari, Fataneh Pharmaceutical Sciences Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Hosseinzadeh, Leila Research Center of Oils and Fats - Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract :
In this study, we evaluated the cytotoxic potential of fractions (F1-F5) isolated
from hexane extract of the seeds of N. sativa on human ovarian carcinoma cell
line, A2780. F2 showed an outstanding potent cytotoxic effect against A2780
cells. Next, this fraction was purified to obtain six sub-fractions (SF1-SF6) and
their cytotoxic effects were then evaluated. The obtained results showed that
SF2 had strong cytotoxic effect against A2780 cell line. The effective subfraction
(SF2) was determined to be linoleic acid (LA) according to
spectroscopic analyses. In the next set of experiments, the apoptotic potentials
of LA were investigated. Induction of apoptosis by LA was accompanied by an
increase in activation of caspase-3, -9 and reduction in mitochondrial
membrane potential (MMP) in A2780 cells. It can be concluded that LA,
inhibited the growth of human ovarian carcinoma cells, A2780 and induced
mitochondrial-related apoptosis.
Keywords :
A2780 Cells , Apoptosis , Cytotoxicity , Linoleic Acid , N. sativa
