Author/Authors :
Farzanegan Gharabolagh, Amin Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University,Tehran, Iran , Sabahi, Farzaneh Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University,Tehran, Iran , Karimi, Mohsen Department of Medical Neuroscience - Center for Molecular Medicine - Karolinska Institute, Stockholm, Sweden , Kamalinejad, Mohammad Faculty of Pharmacy - Shahid Beheshti University of Medical Sciences,Tehran, Iran , Mirshahabi, Hessam Department of Microbiology and Immunology - Faculty of Medicine - Zanjan University of Medical Sciences, Zanjan, Iran , Mousavi Nasab, Dawood Departments of Research and Development - Production and Research Complex - Pasteur Institute of Iran , Ahmadi, Nayeb Ali Proteomics Research Center - Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
The current therapies against hepatitis B virus (HBV) infection are only
effective for a number of patients. Mutant viruses that are resistant to
treatment and get created due to the errors in the reverse transcriptase
mechanism cause for prolonged treatments. In this regard, recent studies
have been focused on new therapeutic approaches especially herbal
medications to overcome the problem in resistant patients. The aim of this
study was to evaluate the inhibitory effect of Rhus coriaria L. (Sumac)
aqueous extract on hepatitis B virus replication and HBsAg secretion in
HuH-7 cell line after transfection by HBV. Huh-7 cell line was transfected
by a plasmid (pCH-9/3091) containing HBV genome using Lipofectamine
2000. The aqueous extract of Rhus coriaria L.was prepared and its
cytotoxicity on transfected cells was assessed by MTT assay. The
inhibitory effect of the aqueous extract of Rhus coriaria L.on release of
HBsAg as a consequence of HBV replication was measured by ELISA. Our
results showed a significant lower concentration of HBsAg after exposure
to Rhus coriaria L.extract compared to the untreated control group and
positive control group treated with Lamivudine of HBV transfected Huh-7
cell line. This study represents the inhibitory effect of sumac aqueous
extract on multiplication of HBV and its antigen secretion.
Keywords :
Huh-7 cells , HBsAg , Sumac , Hepatitis B virus Rhus coriaria L
