Other language title :
فعاليت هاي ضد ميكروبي Coriandrum Sativum L. و اثرهاي آن روي خواص ميكروبيولوژيكي ماست
Title of article :
Antimicrobial Activity of Coriandrum sativum L. and Its Effect on Microbiological Properties of Yoghurt
Author/Authors :
Gundogdu, E Department of Food Engineering - Faculty of Engineering and Natural Sciences - University of Gümüşhane - 29100 Gümüşhane, Turkey , Tanriverdi, E Department of Food Engineering - Faculty of Agriculture - University of Selcuk - 42250 Konya, Turkey , Yildiz, H Department of Food Engineering - Faculty of Engineering and Architecture - University of Nevsehir Haci Bektas Veli - 50300 Nevsehir, Turkey
Abstract :
In this study, the antimicrobial effect of Coriandrum sativum L. leaves against 30
different foodborne microorganisms, including 20 bacteria, 7 fungi, and 3 yeast species
was tested, and some microbiological properties of yoghurt produced with lyophilized C.
sativum L. were investigated. Yoghurts were prepared at three different concentrations of
C. sativum L. powder (0.1, 0.2, and 0.3% w/w) and stored at 4°C for 21 days. Ethanol
extract of the fresh plant showed antimicrobial activity against only 9 bacteria and 1 yeast
with different inhibition zone diameter changing from 8 to 12 mm, but had no effect on
moulds used in this study. The addition of C. sativum L. had no statistical significance on
the growth of yoghurt bacteria compared with the control sample and they remained over
8 log CFU g-1 in all yoghurts throught the storage period. On the other hand, an increase
was observed in the number of Lactobacillus bulgaricus and a decrease in the number of
Streptococcus thermophilus depending on the concentration of C. sativum L., but this was
not statistically significant (P> 0.05). However, significant differences in yeast and mould
counts were observed (P< 0.05). Yeasts and moulds were not detected in any of the
samples containing C. sativum L. powder, whereas the counts of yeast and mould
increased in control throught the storage period. These results show that the powder of C.
sativum L. leaves can be used an agent for preventing the growth of yeast and mould,
which are shelf-life and quality spoilage microorganisms of yoghurt.
Farsi abstract :
در اﯾﻦ ﭘﮋوﻫﺶ، اﺛﺮﻫﺎي ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﺑﺮگ Coriandrum sativum L. روي 30 ريز جاندار ﻣﯿﮑﺮوارﮔﺎﻧﯿﺰم( ﻧﺎﺷﯽ از ﻏﺬا )food borne ﺷﺎﻣﻞ 20 ﺑﺎﮐﺘﺮي، 7 ﻗﺎرچ، و ﺳﻪ ﮔﻮﻧﻪ ﻣﺨﻤﺮ آزﻣﻮد ﺷﺪ و ﺑﺮﺧﯽ ﺧﻮاص ﻣﯿﮑﺮوﺑﯿﻮﻟﻮژﯾﮑﯽ ﻣﺎﺳﺖ ﺗﻮﻟﯿﺪ ﺷﺪه ﺑﺎ ﺧﺸﮏ ﮐﺮدن اﻧﺠﻤﺎدي (lyophilized) C. sativum L. ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ. ﻣﺎﺳﺖ ﻫﺎ ﺑﺎ ﺳﻪ ﻏﻠﻈﺖ از ﭘﻮدر ،%0/2 ،% 0/1) C. sativum L. 0/3%( ﺗﻬﯿﻪ ﺷﺪ و در 4 درﺟﻪ ﺳﺎﻧﺘﯽ ﮔﺮاد ﺑﻪ ﻣﺪت 21 روز ﻧﮕﻬﺪاري ﺷﺪ. ﻋﺼﺎره اﺗﺎﻧﻮل از ﮔﯿﺎه ﺗﺎزه داراي ﻓﻌﺎﻟﯿﺖ ﻫﺎي ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻋﻠﯿﻪ ﻓﻘﻂ 9 ﺑﺎﮐﺘﺮي و ﯾﮏ ﻣﺨﻤﺮ ﺑﻮد ﮐﻪ ﻗﻄﺮ ﻧﺎﺣﯿﻪ ﺑﺎزدارﻧﺪﮔﯽ ) inhibition zone( ﺑﯿﻦ 8 ﺗﺎ 12 ﻣﯿﻠﯽ ﻣﺘﺮ ﺗﻐﯿﯿﺮ ﻣﯽ ﮐﺮد وﻟﯽ ﻫﯿﭻ اﺛﺮي روي ﮐﭙﮏ ﻫﺎي اﺳﺘﻔﺎده ﺷﺪه در اﯾﻦ ﭘﮋوﻫﺶ ﻧﺪاﺷﺖ. اﻓﺰودن C. sativum L. در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻫﯿﭽﮕﻮﻧﻪ ﺗﺎﺛﯿﺮ ﻣﻌﻨﺎدار آﻣﺎري روي رﺷﺪ ﺑﺎﮐﺘﺮي ﻫﺎي ﻣﺎﺳﺖ ﻧﺪاﺷﺖ و آﻧﻬﺎ در ﺣﺪي ﺑﯿﺶ از 8 log CFU/g در ﺗﻤﺎم ﻣﺎﺳﺖ ﻫﺎ در ﻃﯽ دوره اﻧﺒﺎري ﺛﺎﺑﺖ ﻣﺎﻧﺪﻧﺪ. از ﺳﻮي دﯾﮕﺮ، اﻓﺰاﯾﺸﯽ در ﺷﻤﺎرش Lactobacillus bulgaricus و ﮐﺎﻫﺸﯽ در Streptococcus thermophilus ﻣﺸﺎﻫﺪه ﺷﺪ ﮐﻪ ﺑﻪ ﻏﻠﻈﺖ .Csativum L. واﺑﺴﺘﮕﯽ داﺷﺖ وﻟﯽ اﯾﻦ اﻣﺮ از ﻧﻈﺮ آﻣﺎري ﻣﻌﻨﺎدار ﻧﺒﻮد)p>0.05(. ﺑﺎ اﯾﻦ وﺟﻮد، ﺗﻔﺎوت ﻫﺎي ﻣﻌﻨﺎداري ﺑﯿﻦ ﺷﻤﺎرش ﻣﺨﻤﺮﻫﺎ و ﮐﭙﮏ ﻫﺎ ﻣﺸﺎﻫﺪه ﺷﺪ )p<0.05(. در ﻫﯿﭻ ﯾﮏ از ﻧﻤﻮﻧﻪ ﻫﺎﯾﯽ ﮐﻪ داراي ﭘﻮدر C. sativum L. ﺑﻮدﻧﺪ ﻣﺨﻤﺮ و ﮐﭙﮏ ﺷﻨﺎﺳﺎﯾﯽ ﻧﺸﺪ، در ﺣﺎﻟﯿﮑﻪ ﺷﻤﺎرش ﻣﺨﻤﺮ ﻫﺎ و ﮐﭙﮏ ﻫﺎ در ﺗﯿﻤﺎر ﺷﺎﻫﺪ در ﻃﯽ دوره اﻧﺒﺎرداري اﻓﺰاﯾﺶ ﯾﺎﻓﺖ. اﯾﻦ ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽ دﻫﺪ ﮐﻪ ﭘﻮدر ﺑﺮگ .C sativum L. را ﻣﯽ ﺗﻮان ﺑﻪ ﻋﻨﻮان ﻋﺎﻣﻠﯽ ﺑﺮاي ﺟﻠﻮﮔﯿﺮي از رﺷﺪ ﻣﺨﻤﺮ و ﮐﭙﮏ ﮐﻪ رﯾﺰﺟﺎﻧﺪاران ﻣﺨﺮب ﮐﯿﻔﯿﺖ ﻣﺎﺳﺖ در دوره اﻧﺒﺎرداري ﻫﺴﺘﻨﺪ ﺑﻪ ﮐﺎر ﺑﺴﺖ
Keywords :
Yoghurt bacteria , Spoilage microorganisms , Shelf-life , Foodborne microorganisms
Journal title :
Journal of Agricultural Science and Technology (JAST)