Title of article :
Role of Oxidative Stress in Modulating Unfolded Protein Response Activity in Chronic Myeloid Leukemia Cell Line
Author/Authors :
Bazi, Ali Cancer Molecular Pathology Research Center - Imam Reza Hospital - Faculty of Medicine - Mashhad University of Medical Sciences - Mashhad, Iran , Keramati, Mohammad Reza Cancer Molecular Pathology Research Center - Imam Reza Hospital - Faculty of Medicine - Mashhad University of Medical Sciences - Mashhad, Iran , Gholamin, Mehran Division of Human Genetics - Immunology Research Center - Avicenna Research Institute - Mashhad University of Medical Sciences - Mashhad, Iran
Abstract :
Recently, it has been revealed that tyrosine kinase inhibitors (TKIs) act through inducing both
oxidative and endoplasmic reticulum (ER) stress in chronic myeloid leukemia cells. However, ER stress signaling
triggers both apoptotic and survival processes within cells. Nevertheless, mechanisms by which TKIs avoid the
pro-survival effects are not clear. The aim of this study was to evaluate the potential role of oxidative stress in
activity of unfolded protein response (UPR) survival pathway within K562 cell line. Methods: The expression of
UPR survival target genes, Xbp1, and Grp94 (glucose requiring protein 94) was studied in single and combined
exposure to oxidative and ER stress in K562 cell line by quantitative and qualitative PCR. Results: The expression
of UPR-related survival gene Grp94 was hampered by exposing to oxidative stress in cell induced with ER stress.
Conclusion: Interaction of oxidative and ER stress may role as a mediator influencing UPR signaling activity.
Keywords :
Endoplasmic reticulum stress , Oxidative stress , Unfolded protein response
Journal title :
Iranian Biomedical Journal(IBJ)
