Title of article :
Anti-Mutagenicity and Apoptotic Effects of Teucrium polium L. Essential Oil in HT29 Cell Line
Author/Authors :
Hashem-Dabaghian, Fataneh Research Institute for Islamic and Complementary Medicine - Iran University of Medical Sciences, Tehran, Iran , Shojaii, Asie Research Institute for Islamic and Complementary Medicine - Iran University of Medical Sciences, Tehran, Iran , Asgarpanah, Jinous Herbal Pharmacology Research Center - Tehran Medical Sciences Branch Islamic Azad University, Tehran, Iran , Entezari, Maliheh Department of Medical Biotechnology - Tehran Medical Sciences Branch Islamic Azad University, Tehran, Iran
Abstract :
Objectives: Regarding the high prevalence of cancer in Iran and the cytotoxic properties of T. polium, the current study aimed to investigate the cytotoxic and anti-mutagenicity effect of T. polium essential oil (TpEO) on human colon adenocarcinoma cell line (HT29).
Methods: HT29 cells were cultured in L-glutamine, RPMI Sigma (1640), with 10% of FBS (fetal bovine serum). Then, the cultures were incubated with 5% CO2 at 37°C, and MTT assay was used to recognize cell proliferation under the inhibitory influence of TpEO. The cell cycle progression was monitored by Sub-G1 apoptosis assay using flow cytometry. Eventually, the anti-mutagenicity property
was evaluated using the Ames test employing TA100 and exposure to sodium azide as the carcinogenic substance.
Results: The cytotoxic effect of TpEO on HT29 cells was 66.867 1.37 g/mL. Cultured HT29 cells treated with TpEO exhibited morphological
features of apoptosis. TpEO preventive effect was about 78.18%.
Conclusions: This study showed that TpEO may be useful for treating colon cancer.
Keywords :
Teucrium polium , Persian Medicine , Herbal Medicine, HT29 Cell Line , Mutagenicity , Anti-Neoplastic Agents
Journal title :
Jundishapur Journal of Natural Pharmaceutical Products (JJNPP)
