Author/Authors :
Vaziri, Siavash Department of Infectious Disease - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Afsharian, Mandana Department of Infectious Disease - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Mansouri, Feizollah Department of Infectious Disease - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Azizi, Mohsen Department of Microbiology - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Nouri, Fatemeh Student Research Committee - Kermanshah University of Medical Sciences, Kermanshah, Iran , Madadi-Goli, Nahid Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran, Iran , Mohseni Afshar, Zainab Department of Infectious Disease - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Zamanian, Mohammad Hossein Department of Infectious Disease - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Alvandi, Amirhooshang Department of Microbiology - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Ahmadi, Kamal Department of Microbiology - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract :
Background: Assessment of bacteria such as Klebsiella pneumonia has shown that Plasmid-mediated quinolone resistance (PMQR) a ects antibiotics resistance (e.g., quinolones).
Objectives: We studied the prevalence of qnr and aac(6’)Ib-cr genes in extended-spectrum beta-lactamase (ESBL)-producing K. pneu-monia strains isolated from burn wounds of patients in the city of Kermanshah, Iran.
Methods: This descriptive-analytical study was conducted on 126 K. pneumonia strains isolated collected from burn wounds. Bio-chemical tests were used to detect the strains. The frequency of the ESBL-producing isolates was determined by phenotypic tests of the combination disk (CD) method after determining the antibiotic susceptibility pattern of the isolates through the Kirby-Bauer disc di usion test. The prevalence of the qnr and aac(6’)-Ib-cr genes was determined using their special primers as well as polymerase chain reaction (PCR).
Results: Of the 126 K. pneumonia isolates, 52 (41.3%) were identified as ESBL-producing strains. ESBL-producing isolates showed higher resistance against antibiotics than non-ESBL-producing ones. PMQR relevance and resistance to ciprofloxacin were, respectively, determined at 80.76% and 59.6%. The most frequent gene was aac(6’)-Ib-cr (n = 70, 55.6%), followed by the qnrB (n = 44, 34.9%). Conclusions: This study showed a high prevalence of qnr genes in ESBL-producing K. pneumonia isolates and antibiotic resistance. Given the horizontal transmission of antibiotic resistance genes among bacteria by mobile genetic elements, timely identification of infections caused by ESBL-producing and antimicrobial-resistant K. pneumonia strains is of paramount importance.
