Title of article :
Analysis of Single Nucleotide Polymorphisms in HLA-DRA, IL2RA, and HMGB1 Genes in Multiple Sclerosis
Author/Authors :
Mahboudi , Fereidoun Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran - Tehran, Iran , Asouri, Mohsen Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran - Tehran, Iran , Karimpoor, Morteza Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran - Tehran, Iran , Alinejad Rokni, Hamid Systems Biology and Health Data Analytics Lab - the Graduate School of Biomedical Engineering - UNSW Sydney - 2052 - NSW, AU , Sahraian, Mohammad Ali Multiple Sclerosis Research Center - Neuroscience Institute - Tehran University of Medical Sciences - Tehran, Iran , Doosti, Rozita Multiple Sclerosis Research Center - Neuroscience Institute - Tehran University of Medical Sciences - Tehran, Iran , Fattahi, Sadegh North Research Center - Pasteur Institute of Iran - Amol, Iran , Lotfi, Maryam North Research Center - Pasteur Institute of Iran - Amol, Iran , Motamed, Nima Department of Social Medicine - Zanjan University of Medical Sciences - Zanjan, Iran , Rahimi, Hamzeh Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran - Tehran, Iran , Moslemi, Azam Department of Biostatistics - Faculty of Medical Scienc- Arak University of Medical Sciences - Arak, Iran , Akhavan-Niaki, Haleh Zoonoses Research Center - North Research Center - Pasteur Institute of Iran - Amol, Iran
Abstract :
Multiple sclerosis (MS) is a common demyelinating neurodegenerative disorder with
significant heritability. Previous studies have associated genetic variants in human leukocyte antigen (HLA)
complex, IL2RA, and HMGB1 genes with the pathophysiology of MS.
Methods: In order to investigate the gene association in the Iranian population, we performed a genotyping study
of 36 variants in the mentioned genes using Sanger sequencing in 102 MS patients and 113 healthy controls.
Results: Our results identified significant associations as well as significant allele frequency differences in
some of the studied single-nucleotide polymorphisms including rs4935356, rs3177928, and rs7197 from
HLA-DRA gene, and rs12722489 and rs12722490 variants from IL2RA gene (p< 0.05). Moreover, the strong
linkage disequilibrium of two common haplotypes was estimated from the HLA-DRA gene.
Conclusions: This association study may suggest the role of these polymorphisms in the genetic
susceptibility of MS in the Iranian population and would facilitate the recognition of causative variants
in this disease.
Keywords :
Polymorphism , Multiple sclerosis , HLA-DRA - IL2RA , HMGB1
