Author/Authors :
Salimi, Maryam Department of Biology and Anatomical Sciences - Faculty of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Norouzian, Mohsen Department of Biology and Anatomical Sciences - Faculty of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Shirazi, Abolfazl Reproductive Biotechnology Research Center - Avicenna Research Institute - ACECR - Tehran, Iran , Edalatkhah, Haleh Reproductive Biotechnology Research Center - Avicenna Research Institute - ACECR - Tehran, Iran , Mehravar, Maryam Reproductive Biotechnology Research Center - Avicenna Research Institute - ACECR - Tehran, Iran , Mehrazar, Mohammad Mahdi Reproductive Biotechnology Research Center - Avicenna Research Institute - ACECR - Tehran, Iran , Jafari, Ameneh Student Research Committee - School of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Majidi, Mohammad Food and Drug Laboratory Research Center - Food and Drug Organization - MOH & ME - Tehran, Iran
Abstract :
Currently, the efficient production of chimeric mice and their survival are still challenging.
Recent researches have indicated that preimplantation embryo culture media and manipulation lead to
abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene expression
pattern. This investigation was designed to evaluate the relationship between the methylation state of histone
H3 and H19/Igf2 expression in mice chimeric blastocysts.
Methods: Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs-
GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a
micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by
immunocytochemistry and q-PCR, respectively, in blastocysts.
Results: Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and
greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2
expression was less, but not significantly so, in chimeric than in control blastocysts.
Conclusions: Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change
H19/Igf2 expression in chimeric blastocysts.
