Title of article
Variations of Histone Acetyltransferase 300 in Patients with Human Papillomavirus Type 6-Associated Anogenital Warts
Author/Authors
Zakeri, Mana Department of biology - Islamic Azad University - Tehran Medical Branch , Mohebbi, Alireza epartment of Microbiology - School of Medicine - Golestan University of Medical Sciences , Sana Askari, Fatemeh Department of Microbiology - School of Medicine - Golestan University of Medical Sciences , Yasaghi, Mohammad Department of Biology - Islamic Azad University of Tehran - Medical Branch
Pages
7
From page
10
To page
16
Abstract
Background and objectives: Pathogenesis of human papillomaviruses (HPVs) is controlled by viral and host factors, among which human histone acetyltransferase p300 (EP300) plays an important role. This study aimed to examine single nucleotide polymorphisms (SNPs) at the EP300 binding site in patients with HPV-associated anogenital wart.
Methods: After DNA extraction, polymerase chain reaction was performed to determine HPV genotypes. Human p300 was amplified to detect SNPs using Sanger sequencing.
Results: Overall, 35.3% of HPV-6-positive patients had Ile997Val substitution at the EP300 binding site. Another SNP containing A to G point mutation leading to Glu983Gly was also detected. In addition, Ile997Val substitution of EP300 was frequently observed in the patients.
Conclusion: Our findings suggest that the EP300 genotype Ile/Val can be involved in HPV-6 pathogenesis. In addition, we introduced a new genotype (Glu983Gly) at the EP300 bromodomain site, which requires further investigation.
Keywords
Human Papilloma Virus , Anogenital warts , Histone acetyltransferase p300 , Polymorphism , EP300 , Single Nucleotide Polymorphism
Journal title
Medical Laboratory Journal
Serial Year
2020
Record number
2525813
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