Title of article :
Production and Solubility of Ectoine: Biochemical and Molecular Dynamics Simulation Studies
Author/Authors :
Rezazadeh Mofradnia ، Soheil Department of Chemical Engineering - Faculty of Engineering - Islamic Azad University, North Tehran Branch , Ashouri ، Reihaneh Department of Environment - Faculty of Environment and Energy - Islamic Azad University, Science and Research Branch , Abtahi ، Najmeh Department of Chemical Science - Faculty of Science Technology - Islamic Azad University, North Tehran Branch , Yazdian ، Fatemeh Department of Life Science Engineering - Faculty of New Science Technology - University of Tehran , Rashedi ، Hamid Biotechnology Group - School of Chemical Engineering, College of Engineering - University of Tehran , Sheikhpour ، Mojgan Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC) - Pasteur Institute of Iran , Ashrafi ، Fatemeh Department of Chemical Science - Faculty of Science Technology - Islamic Azad University, North Tehran Branch
Abstract :
In this study, ectoine is produced by Streptomyces.sp IBRC-M PTCC 10615. Fermentation parameters such as flow regime, gas hold up, mass transfer coefficient, and mixing time were optimized by statistical analysis. Streptomyces. sp produced a maximal ectoine concentration of 270 mmol/kg at optimal conditions of ectoine and L-aspartic acid. Also, the amount of mass transfer, gas hold up, and mixing time were determined 0,41/s ,0.3, and 40 s, respectively. The amount of ectoine was measured by HPLC. Furthermore, Molecular Dynamics (MD) simulation was used for studying the solubility of ectoine in aqueous media. Equilibrium data such as temperature, potential energy, and volume graphs showed that the solubility of ectoine is 25 % more than glycerol. Also, all the achieving graphs from the equilibrium of simulation were confirmed the appropriate structure of the system.
Keywords :
Ectoine , Streptomyces , Molecular dynamic , Thermodynamic principles
Journal title :
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Journal title :
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
