Investigation of the Effects of Pre-Incubation Formaldehyde Fumigation on the Tracheal Epithelium of Chicken Embryos and Chicks

This study aimed to evaluate the effects of pre-incubation formaldehyde fumigation on the tracheal epithelium of chicken embryos and chicks. Throughout the study pre-incubation formaldehyde fumigation was applied to 18-day-old embryos and 1-day oldchicks only once, at 1 of 2 different concentrations (3×, 42 ml of formalin and 21 g of potassium permanganate per m3 and4×, 56 ml of formalin and 28 g of potassium permanganate per m3) for 1 of 2 different durations (20 min and 40 min). Trachealsamples were taken from 18-day-old embryos and 1-day-old chicks, and the tracheal epithelial cells were examined with transmission electron microscopy (TEM). According to TEM, after fumigation cilia in the epithelial cells were shorter and fewer in number, and vacuolisation, swelling of the mitochondria, and spoiling of cristae were observed in the subjects, which varied according to fumigation concentration and duration.