Author/Authors :
DIKMEN, Z. Günnur Hacettepe University - Faculty of Medicine - Department of Biochemistry, TURKEY , DIKMEN, Z. Günnur University of Texas Southwestern - Department of Cell Biology, USA , GELLERT, Ginelle University of Texas Southwestern - Department of Cell Biology, USA , DOGAN, Pakize Hacettepe University - Faculty of Medicine - Department of Biochemistry, TURKEY , MASON, Ralph University of Texas Southwestern - Department of Radiology, USA , ANTICH, Peter University of Texas Southwestern - Department of Radiology, USA , RICHER, Edmond University of Texas Southwestern - Department of Radiology, USA , WRIGHT, Woodring E University of Texas Southwestern - Department of Cell Biology, USA , SHAY, Jerry W University of Texas Southwestern - Department of Cell Biology, USA
Abstract :
Bioluminescent imaging (BLI) is particularly well suited for imaging small animals, and it can be readily used by scientistswho are already routinely using the luciferase gene as a reporter in cell-based assays. This strategy relies on ATP and an O2dependent photochemical reaction between luciferin and luciferase, resulting in the release of photons from only live cells. In BLI,the intensity of the light detected by the device is proportional to the intensity of light emitted and the relationship is quantitative.This study was performed to determine the sensitivity of BLI for the imaging of lung metastasis and breast cancer models in additionto primary flank tumors. A549 lung cancer and MDA-MB 231 breast cancer cell lines were transfected with a lentiviral constructcontaining the luciferase gene and transfected cells were used as xenograft animal models. BLI was used to monitor tumordevelopment and growth. Noninvasive bioluminescent imaging in vivo provides easy visualization of the tumor size and location sothat imaging results can be used as an indicator of the treatment effectiveness, potentially accelerating the optimization of cancertreatment protocols
