Author/Authors :
KARAHAN, Zeynep Ceren Ankara Üniversitesi - Faculty of Medicine - Department of Medical Microbiology, TURKEY , KOYUNCU, Esra Ankara Üniversitesi - Faculty of Medicine - Department of Medical Microbiology, TURKEY , DOLAPÇI, Istar Ankara Üniversitesi - Faculty of Medicine - Department of Microbiology and Clinical Microbiology, TURKEY , ARIKAN AKAN, Özay Ankara Üniversitesi - Faculty of Medicine - Ibn-i Sina Hospital Central Bacteriology Laboratory, TURKEY , CAN, Füsun Baskent University - Faculty of Medicine - Department of Medical Microbiology, TURKEY , TEKELI, Alper Ankara Üniversitesi - Faculty of Medicine - Department of Medical Microbiology, TURKEY
Abstract :
Aim: Trichosporon infections are associated with a wide spectrum of clinical manifestations. Trichosporon asahii is the most important species in the genus. Our aim was to evaluate the genotypic relatedness of urinary T. asahi i strains isolated from immunocompetent patients during a hospital outbreak.Materials and methods: Twenty-three T. asahi i isolates obtained from 15 patients hospitalized in different wards of Ankara University Ibn-i Sina Hospital between July 2004 and April 2005 were studied. T. asahi i was identified by API 20CAUX (BioMérieux Vitek, Hazelwood, USA) system and confirmed by genus and species specific polymerase chain reactions. Genetic relatedness of the isolates was evaluated by randomly amplified polymorphic DNA (RAPD) and repetitive element PCR (REP-PCR) analyses. Results: RAPD analysis divided the isolates into 5 groups. The results of REP-PCR correlated well with the data obtained by RAPD analysis, although this method produced 3 different groups. Conclusion: In this study of urinary trichosporonosis, 19 of the 23 isolates were found to be genotypically related,suggesting that a single genotype was prevalent in our hospital environment. The genotypically unrelated 4 isolates were probably of endogenous origin. RAPD and REP-PCR analyses can be used for genotyping T. asahii strains.
