Author/Authors :
BOZER, Mikdat Turgut Özal University - Faculty of Medicine - Department of General Surgery, Turkey , AŞIK, Fatma Turgut Özal University - Faculty of Medicine - Department of General Surgery, Turkey , ACAR, Muradiye Turgut Özal University - Faculty of Medicine - Department of Medical Genetics, Turkey , HALTAŞ, Hacer Turgut Özal University - Faculty of Medicine - Department of Pathology, Turkey , YENİDÜNYA, Sibel Turgut Özal University - Faculty of Medicine - Department of Pathology, Turkey , CANBAL, Metin Turgut Özal University - Faculty of Medicine - Department of Family Practice, Turkey , TOPÇU, Vehap Zekai Tahir Burak Woman’s Health Training and Research Hospital - Department of Medical Genetics, Turkey , YİĞİTOĞLU, Muhammet Ramazan Turgut Özal University - Faculty of Medicine - Department of Biochemistry, Turkey , GÜNDÜZ, Mehmet Turgut Özal University - Faculty of Medicine - Department of Medical Genetics, Turkey , GÜNDÜZ, Esra Turgut Özal University - Faculty of Medicine - Department of Medical Genetics, Turkey , HİROHATA, Satoshi Okayama University - Graduate School of Medicine, Dentistry, and Pharmacological Sciences - Department of Molecular Biology and Biochemistry, Japan , DEMİRCAN, Kadir Okayama University - Graduate School of Medicine, Dentistry, and Pharmacological Sciences - Department of Molecular Biology and Biochemistry, Japan , DEMİRCAN, Kadir Turgut Özal University - Faculty of Medicine - Department of Medical Biology, Türkiye
Abstract :
Aim: We investigated the effect of the number of cytosine-adenine (CA) repeats in the ADAMTS9 promoter region on breast cancer lymphatic metastasis. Materials and methods: Thirty-one postoperative breast cancer patients were selected and examined retrospectively. The patients were classified into 2 groups: metastatic or nonmetastatic. Thirty healthy women were selected as the control group, and their peripheral blood was obtained. Following DNA isolation from the cancer tissue specimens and peripheral blood, the promoter region of the ADAMTS9 gene was directly sequenced and the number of CA repeats was determined. Results: The number of CA repeats ranged between 19 and 21 in the control and metastatic groups. However, in the nonmetastatic group, the number of CA repeats ranged between 17 and 18. This difference in the median number of CA repeats between the control group and the nonmetastatic group was statistically significant. Conclusion: A potential relationship may exist between lymphatic metastasis in breast cancer and the number of CA repeats in the promoter region of the ADAMTS9 gene. Our study indicates a potential association between the number of CA microsatellite repeats in the promoter region of the ADAMTS9 gene and breast cancer lymphatic metastasis.
Keywords :
Breast cancer , lymphatic metastasis , ADAMTS9 gene , promoter region , dinucleotide repeats
