Author/Authors :
AY, Hakan Gülhane Haydarpaşa Military Training Hospital - Department of Undersea and Hyperbaric Medicine, Turkey , YÜKSEL, Ramazan Gülhane Military Medical Academy - Department of Physiology, Turkey , ŞİMŞEK, Kemal Gülhane Military Medical Academy - Department of Undersea and Hyperbaric Medicine, Turkey , TOPAL, Turgut Gülhane Military Medical Academy - Department of Physiology, Turkey , YEŞİLYURT, Özgur Gülhane Military Medical Academy - Department of Medical Pharmacology, Turkey , ÖTER, Şükrü Gülhane Military Medical Academy - Department of Physiology, Turkey , KORKMAZ, Ahmet Gülhane Military Medical Academy - Department of Physiology, Turkey , ÖZLER, Mehmet Gülhane Military Medical Academy - Department of Physiology, Turkey , UYSAL, Bülent Gülhane Military Medical Academy - Department of Physiology, Turkey , YAMAN, Halil Gülhane Military Medical Academy - Department of Medical Biochemistry, Turkey , BEDİR, Orhan Gülhane Military Medical Academy - Department of Blood Education Center and Blood Bank, Turkey , BILGIÇ, Hayati Gülhane Military Medical Academy - Department of Pulmonary Medicine, Turkey
Abstract :
Since blood bags have the ability for diffusion of gases, we investigated whether hyperbaric oxygen (HBO) exposure affects several vital parameters of stored blood. Materials and methods: Bloods obtained from the same persons were used as both control and HBO groups and stored in pediatric bags with citrate-phosphate-dextrose solution. HBO administration was performed at 2.5 atm for 90 min, started 1 day after blood collection and repeated every 2 days for a total of 10 times. The study was terminated on the 21st day. Complete blood count, glucose, pH, and osmotic fragility values were measured every week. Results: Glucose and pH levels decreased in stored blood. In the HBO-exposed group, these decreases were less than in the control. In addition, mean corpuscular and platelet volumes tended to increase during storing process, but with HBO, these indexes remained lower, near physiologic levels. Another interesting finding of the study was the relative stable osmotic fragility ratio in the HBO group compared to the control blood. Conclusion: HBO exposure has positive effects on pH, stability of erythrocytes, and energy source (glucose) of the medium. Thus, we concluded that HBO may be a useful application for life and quality of stored blood.
