Author/Authors :
TOKAÇ, Mehmet Ministry of Health - Atatürk Training and Research Hospital - Department of Surgery, Turkey , AYDIN, Sevtap Hacettepe University - Faculty of Pharmacy - Department of Pharmaceutical Toxicology, Turkey , TANER, Gökçe Gazi Üniversitesi - Faculty of Science - Department of Biology, Turkey , ÖZKARDEŞ, Alper Bilal Ministry of Health - Dışkapı Yıldırım Beyazıt Education and Research Hospital - Department of Surgery, Turkey , YAVUZ TAŞLIPINAR, Mine Ministry of Health - Dışkapı Yıldırım Beyazıt Education and Research Hospital - Department of Clinical Biochemistry, Turkey , DOĞAN, Mehmet Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital - Department of Clinical Pathology, Turkey , DÜNDAR, Halit Ziya Uludağ University - Faculty of Medicine - Department of Surgery, Turkey , KILIÇ, Mehmet Yıldırım Beyazıt University - Faculty of Medicine - Department of Surgery, Turkey , BAŞARAN, Arif Ahmet Hacettepe University - Faculty of Pharmacy - Department of Pharmacognosy, Turkey , BAŞARAN, Ayşe Nurşen Hacettepe University - Faculty of Pharmacy - Department of Pharmaceutical Toxicology, Turkey
Abstract :
Background/aim: Lycopene, which is suggested to be a potent antioxidant, may play a protective role in diseases related to oxidative stress. In order to understand the effects of lycopene in the pathogenesis of cholestasis, we investigated the effects of lycopene on oxidative stress parameters and DNA damage induced by experimental biliary obstruction in the liver tissues and the lymphocytes of Wistar albino rats. Materials and methods: The animals were randomized into 3 groups. The sham group was subjected to a sham operation, the BDL group was subjected to bile duct ligation (BDL), and the BDL+L group was subjected to BDL and treated with 10 mg/kg body weight of lycopene. After 7 days of treatment, the liver functions, oxidative stress parameters, and DNA damage were evaluated. Results: The lycopene treatment significantly ameliorated the liver function parameters in BDL rats. It significantly reduced malondialdehyde and nitric oxide levels and enhanced reduced glutathione levels and catalase, superoxide dismutase, and glutathione S transferase activities in the BDL rats. The lycopene treatment also decreased DNA damage as assessed by comet assay in the lymphocytes and hepatocytes of the BDL rats. Conclusion: These results suggest that lycopene might have protective effects on acute cholestasis.
Keywords :
Lycopene , cholestasis , DNA damage , oxidative stress , peripheral lymphocytes , liver
