Author/Authors :
ahmadi, t. isfahan university of technology - department of materials engineering, ايران , monshi, a. isfahan university of technology - department of materials engineering, ايران , mortazavi, v. isfahan university of medical sciences - department of operative dentistry, ايران , fathi, m.h. isfahan university of technology - department of materials engineering, ايران , fathi, m.h. isfahan university of medical sciences - department of operative dentistry, ايران , hashemibeni, b. isfahan university of medical sciences - department o f anatomical sciences and molecular biology, ايران , sharifnabi, a. isfahan university of technology - department of materials engineering, ايران , sharifnabi, a. iran university of science and technology - department of metallurgy and materials engineering, ايران
Abstract :
Hydroxyapatite (HA) is perhaps the most attractive material for bone repair, replacement and regeneration due to its chemical composition and crystallographic structure which are similar to those of natural bone mineral. However, replacement of various elements and compounds in HA could improve biological properties of this material. Aim of this study was in vitro bioactivity and cellular behavior evaluation of mechanically activated nanosized Silicon and Magnesium co-doped Fluorapatite (Si-Mg-FA). In vitro bioactivity was evaluated in simulated body fluid (SBF) at 37˚C for up to 28 days. The apatite precipitates were proved with Scanning Electron Microscopy (SEM) and Fourier Transformed Infrared Spectroscopy (FTIR). Cell viability and cell attachment were studied by MTT assay and scanning electron microscopy (SEM). In vitro examinations revealed the amount of bone-like apatite precipitated on Si-Mg-FA nanopowder was significantly higher than FA. The cell culture medium containing Si-Mg-FA showed more cell proliferation and cell viability than FA. It could be concluded that doping Si and Mg into FA improves the bioactivity and cell viability, therefore, Si-Mg-FA has a good potential to be used as bone substitution material.
Keywords :
Mechanical Activation , Si , Mg , Doped Fluorapatite , Nanostructured Materials , In , Vitro Cytotoxicity , In , Vitro Bioactivity , Biomedical Applications
