Title of article :
Efficacy of a Direct Rapid Immunohistochemical Test (DRIT) For Rabies Detection in Nigeria
Author/Authors :
Ehimiyein, A. M. Ahmadu Bello University - Department of Veterinary Medicine, Nigeria , Niezgoda, M. Centers for Disease Control and Prevention, USA , Orciari, L. Centers for Disease Control and Prevention, USA , Osinubi, M. O. V. Centers for Disease Control and Prevention, USA , Ehimiyein, I. O. Gimaf Veterinary Consult, Nigeria , Adawa, D. A. Y. Ahmadu Bello University - Veterinary Teaching Hospital, Nigeria , Abdullahi, S. U. Ahmadu Bello University - Department of Veterinary Medicine, Nigeria , Ogunkoya, A. B. Ahmadu Bello University - Department of Veterinary Medicine, Nigeria , Rupprecht, C. E. The Global Alliance for Rabies Control, USA , Rupprecht, C. E. Ross University - School of Veterinary Medicine, Saint Kitts and Nevis
From page :
101
To page :
107
Abstract :
Rabies is an acute, infectious disease mostly transmitted through bites from an infected animal. Dogs majorly transmit rabies to humans. Human rabies is not curable once clinical signs commence, but can be prevented. The aim of this study was to find an appropriate diagnostic test suitable for use in Nigeria and other developing countries with infrastructural challenges. Thirty dog brain samples collected from dog markets in Kaduna State were analysed using four tests direct fluorescent antibody test (DFA), mouse inoculation test (MIT), Seller’s staining test, the reverse transcriptase-polymerase chain reaction test (RT-PCR) and the direct rapid immunohistochemistry test (dRIT). A total of 15 (50%) of the samples tested positive using DFA, dRIT, RT-PCR and MIT, while the remaining 15 (50%) were negative. The results obtained using these four different tests showed concordance between those that were positive and negative. There was no statistical difference (P 0.05) among the four tests. An appropriate diagnostic test must be prompt, cheap, sensitive, field-based and reliable. The direct rapid immunohistochemistry test is a new diagnostic test established by the Centers for Disease Control and prevention, Atlanta, Georgia, USA. It does not require sophisticated equipment so it can be used on the field especially in the rural areas where most of the rabies exposures occur. It is also as sensitive as the DFA and the result can be obtained within an hour. In Nigeria and most developing countries, prompt diagnosis play a major role in the prevention and control of rabies.
Keywords :
rabies diagnosis , direct rapid immunohistochemistry test , mouse inoculation test , RT , PCR
Journal title :
African Journal of Biomedical Research
Journal title :
African Journal of Biomedical Research
Record number :
2532571
Link To Document :
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