Title of article :
Rapid Separation Method of Ursolic Acid from Pomegranate (Punica granatum L.) Flowers using Normal-Phase Flash Chromatography
Author/Authors :
Sharifiyan, Fatemeh Department of Biology - Payame Noor University (PNU) , Mirjalili, Abbas Imam Khomeini Higher Education Center - Agricultural Research - Education and Extension Organization (AREEO) , Fazilati, Mohammad Department of Biology - Payame Noor University (PNU) , Habibollahi, Saeed Department of Biology - Payame Noor University (PNU) , Poorazizi, Elahe Department of Biochemistry - Najafabad Branch - Islamic Azad University
Abstract :
Ursolic acid (UA) is a major bioactive component in many traditional medicinal plants including pomegranate (Punica granatum L.) flower. In vitro and in vivo studies have both revealed that UA has many important biological functions.In this study, a rapid and efficient procedure for preparation of UA from an Iranian pomegranate flower cultivar using ultrasound-assisted extraction followed by normal-phase flash chromatography (NP-FC) was developed. After one flash chromatography run, the purity of UA reached more than 97% with a total yield of 0.1% of dried pomegranate flower powder. The chemical structure of isolated UA was identified by IR, MS and NMR spectra. This is the first report on isolation of UA from pomegranate flowers. The NP-FC method was shown to be a good method for the purification of UA from pomegranate flowers, and
it can be an excellent basis for large-scale preparation of UA from pomegranate flowers or other plant extracts.
Keywords :
Ursolic acid , Isolation , Pomegranate flowers , Flash chromatography
Journal title :
Quarterly Journal of Applied Chemical Research (JACR)
