Author/Authors :
WANG, Jianhui Sichuan University - College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment, CHINA , WANG, Jianhui Sichuan Academy of Agricultural Sciences - Horticulture Institute, CHINA , WANG, Jianhui Ministry of Agriculture - Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), CHINA , XI, Dehui Sichuan University - College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment, CHINA , LIU, Jianjun Ministry of Agriculture - Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), CHINA , LIU, Jianjun Sichuan Academy of Agricultural Sciences, CHINA , CHEN, Keling Sichuan Academy of Agricultural Sciences - Horticulture Institute, CHINA , CHEN, Keling Ministry of Agriculture - Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), CHINA , LI, Hongwen Sichuan Academy of Agricultural Sciences - Horticulture Institute, CHINA , LI, Hongwen Ministry of Agriculture - Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), CHINA , LIU, Xiao Sichuan Academy of Agricultural Sciences - Horticulture Institute, CHINA , LIU, Xiao Ministry of Agriculture - Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), CHINA , YUAN, Shu Sichuan University - College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment, CHINA , ERCİŞLİ, Sezai Atatürk University - Faculty of Agriculture - Department of Horticulture, Turkey , LIN, Honghui Sichuan University - College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment, CHINA
Abstract :
Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that the parts of the tested grape plants were specifically infected by mild variants.
Keywords :
Vitis vinifera L. × Vitis labrusca L. , Grapevine virus A , PCR , RFLP , cloning and sequencing , RT , PCR
