Title of article :
Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116
Author/Authors :
OBRADOVIĆ, Ana University of Kragujevac - Faculty of Science - Department of Biology and Ecology, Serbia , ŽIŽIĆ, Jovana University of Kragujevac - Faculty of Science - Department of Biology and Ecology, Serbia , TRIŠOVIĆ, Nemanja University of Belgrade - Faculty of Technology and Metallurgy - Department of Organic Chemistry, Serbia , BOŽIĆ, Bojan University of Belgrade - Faculty of Technology and Metallurgy - Department of Organic Chemistry, Serbia , UŠĆUMLIĆ, Gordana University of Belgrade - Faculty of Technology and Metallurgy - Department of Organic Chemistry, Serbia , BOŽIĆ, Biljana University of Belgrade - Faculty of Biology, Institute for Physiology and Biochemistry, Serbia , MARKOVIĆ, Snežana University of Kragujevac - Faculty of Science - Department of Biology and Ecology, Serbia
Abstract :
Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 μM on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O2 .-), nitrites (NO2 -), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O2 .- and an increase in NO2 - concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.
Keywords :
Antioxidants , phenytoin derivatives , free radicals , redox homeostasis
Journal title :
Turkish Journal of Biology
Journal title :
Turkish Journal of Biology
