Title of article :
Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers
Author/Authors :
JARDA, Liliana Babeş-Bolyai University - Al Borza Botanical Garden, Romania , BUTIUC-KEUL, Anca Babeş-Bolyai University - Faculty of Biology and Geology - Department of Molecular Biology and Biotechnology, Romania , HÖHN, Maria Corvinus University - Faculty of Horticultural Science - Department of Botany, Hungary , PEDRYC, Andrzej Corvinus University - Faculty of Horticultural Science - Department of Genetics and Plant Breeding, Hungary , CRISTEA, Victoria Babeş-Bolyai University - Al Borza Botanical Garden, Romania
Abstract :
Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.
Keywords :
ISSR , SSR markers , Dianthus giganteus subsp. banaticus , ex situ conservation , in vitro culture , somaclonal variability
Journal title :
Turkish Journal of Biology
Journal title :
Turkish Journal of Biology
