Title of article :
The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach
Author/Authors :
EROL, Osman Istanbul University - Faculty of Science - Department of Botany, Turkey , KAYA, Hilal Betül Ege University - Faculty of Engineering - Department of Bioengineering, Turkey , ŞIK, Levent Celal Bayar University - Faculty of Arts and Sciences - Department of Biology, Turkey , TUNA, Metin Namık Kemal University - Faculty of Agriculture - Department of Field Crops, Turkey , CAN, Levent Namık Kemal University - Faculty of Arts and Sciences - Department of Biology, Turkey , TANYOLAÇ, Muhammed Bahattin Ege University - Faculty of Engineering - Department of Bioengineering, Turkey
From page :
48
To page :
62
Abstract :
In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 (M-CAA/E-AGC) to 0.512 (M-CAT/E-AAG) and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. These findings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies.
Keywords :
Amplified fragment length polymorphism , Crocus , genetic diversity , genetic structure , nuclear DNA content
Journal title :
Turkish Journal of Biology
Journal title :
Turkish Journal of Biology
Record number :
2534229
Link To Document :
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