Author/Authors :
BALTA, Handan Istanbul University - Faculty of Science - Department of Biology, Turkey , KARAKAŞ METİN, Özge TÜBİTAK Marmara Research Center - Genetic Engineering and Biotechnology Institute, Turkey , ŞENTÜRK AKFIRAT, Funda Gebze Institute of Technology - Faculty of Science - Department of Molecular Biology and Genetics, Turkey , ERTUĞRUL, Fahriye TÜBİTAK - Agriculture, Forestry and Veterinary Research Group, Turkey , HASANÇEBİ, Semra TÜBİTAK Marmara Research Center - Genetic Engineering and Biotechnology Institute, Turkey , AYDIN, Yıldız Marmara University - Faculty of Science and Arts - Department of Biology, Turkey , AKAN, Kadir Field Crop Research Institute, Turkey , MERT, Zafer Field Crop Research Institute, Turkey , TÜRET, Müge Bogazici University - Faculty of Arts and Sciences - Department of Molecular Biology and Genetics, Turkey , ALTINKUT UNCUOĞLU, Ahu Marmara University - Faculty of Engineering - Department of Bioengineering, Turkey
Abstract :
An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.
Keywords :
AFLP , bread wheat , disease resistance , SCAR , yellow rust
