Title of article :
Activity of antioxidant enzymes in the liver of wild boars (Sus scrofa) from a selenium-deficient area depending on sex, age, and season of the year
Author/Authors :
JANKOWIAK, Dorota West Pomeranian University of Technology - Department of Immunology, Microbiology, and Physiological Chemistry, Poland , PILARCZYK, Renata West Pomeranian University of Technology - Laboratory of Biostatistics, Poland , DROZD, Radosław West Pomeranian University of Technology - Department of Immunology, Microbiology, and Physiological Chemistry, Poland , PILARCZYK, Bogumiła West Pomeranian University of Technology - Department of Animal Reproduction, Biotechnology, and Environmental Hygiene, Poland , TOMZA-MARCINIAK, Agnieszka West Pomeranian University of Technology - Department of Animal Reproduction, Biotechnology, and Environmental Hygiene, Poland , WYSOCKA, Gabriela West Pomeranian University of Technology - Faculty of Environmental Studies and Agriculture - Department of Horticulture, Laboratory of Storage and Processing, Poland , RZĄD, Izabella University of Szczecin - Department of Ecology and Environmental Protection, Poland , DROZD, Arleta Pomeranian Medical University - Department of Biochemistry and Human Nutrition, Poland , KUBA, Jarosław West Pomeranian University of Technology - Department of Animal Reproduction, Biotechnology, and Environmental Hygiene, Poland
Abstract :
The aim of this study was to compare the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and total and selenium-dependent glutathione peroxidase (total GSH-Px and Se-GSH-Px) in the liver of wild boars (Sus scrofa) selected from a selenium-deficient area with respect to season of the year, sex, and age. Only season had a significant effect on liver Se content. Liver selenium concentration in the wild boars was highest in spring (0.292 μg/g wet weight) and lowest in winter (0.185 μg/g wet weight) (P 0.05). The highest mean activity of total GSH-Px, Se-GSH-Px, and CAT (404.0, 309.9, and 1170.0 U/mg protein, respectively) was found in the spring season, and the respective highest mean activity of SOD was found during autumn (31.5 U/mg protein). Females showed markedly higher mean activities of total GSH-Px (388.2 and 314.7 U/mg protein) and CAT (1151.0 and 765.8 U/mg protein) compared to males. In comparison to other groups of animals, those aged between 1 and 1.5 years were characterized by higher mean activity of total GSH-Px and Se-GSH-Px, and those over 2 years of age showed higher activity of CAT and SOD. The analysis showed that the strongest oxidative stress and the greatest risk of peroxidative damage to the liver occurred during the winter.
Keywords :
Liver , wild boar , selenium , antioxidant enzymes , seasons of the year
