High Efficiency Somatic Embryogenesis from Immature Zygotic Embryos of Grapevine: The Effect of Genotype, Media, 2,4-D, and Incubation Conditions

Immature zygotic embryos of 41 B grape rootstock (V. vinifera L. cv. ‘Chasselas’ × V. berlandieri) and Yalova ‹ncisi (V.vinifera L.) were cultured on MS, NN, and B5 media supplemented with 0.5, 1, 2, and 4 mg l^-1 2,4-D at 16/8 h light/darkphotoperiod and complete darkness. For 41 B rootstock, the highest somatic embryo formation was obtained from dark conditionon B5 media containing 0.5 and 1 mg l^-1 2,4-D with the rates of 30% and 28.9%, respectively. Yalova ‹ncisi zygotic embryosproduced 5% somatic embryos only at 16/8 h light/dark photoperiod of MS supplemented with 1 mg l^-1 2,4-D, and also in 16/8 hlight/dark photoperiod and complete darkness somatic embryos were produced at the rates of 6.3% and 2.3%, respectively, incultures of NN containing 0.5 mg l^-1 2,4-D. After 8 months of culture, 559 embryos at torpedo stage were identified on B5 + 1mg l^-1 2,4-D + dark cultures of 41 B and 912 embryos at torpedo stage were identified on NN + 0.5 mg l^-1 2,4-D + light culturesof Yalova ‹ncisi. The highest germination and plantlet conversion rates were obtained from 41 B on free NN medium (58% and75%, respectively) and from Yalova ‹ncisi on free MS medium (77.4% and 45%, respectively). Of the regenerated somatic plantlets,91.9% were successfully transferred to soil.