Title of article :
Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.
Author/Authors :
LI, Tian Ministry of Agriculture, Shandong Academy of Agricultural Science - Hi-Tech Research Center - Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops and Key Laboratory of Crop GeneticImprovement and Biotechnology, Huanghuaihai, China , LI, Tian Shandong Normal University - College of Life Science, China , LI, Tian Binzhou University - Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, China , PENG, Zhen-Ying Ministry of Agriculture, Shandong Academy of Agricultural Science - Hi-Tech Research Center - Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops and Key Laboratory of Crop GeneticImprovement and Biotechnology, Huanghuaihai, China , BI, Yu-Ping Shandong Normal University - College of Life Science, China , BI, Yu-Ping Ministry of Agriculture, Shandong Academy of Agricultural Science - Hi-Tech Research Center - Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops and Key Laboratory of Crop GeneticImprovement and Biotechnology, Huanghuaihai, China , FAN, Zhong-Xue Ministry of Agriculture, Shandong Academy of Agricultural Science - Hi-Tech Research Center - Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops and Key Laboratory of Crop GeneticImprovement and Biotechnology, Huanghuaihai, China
From page :
517
To page :
526
Abstract :
V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash.
Keywords :
Arizona ash , expression analysis , molecular cloning , salt stress , V , myb avian myeloblastosis viral oncogene homolog
Journal title :
Turkish Journal of Agriculture and Forestry
Journal title :
Turkish Journal of Agriculture and Forestry
Record number :
2534817
Link To Document :
بازگشت