Author/Authors :
AMMAD, Faiza Superior Normal School - Department of Biology, Algeria , AMMAD, Faiza University of Blida - Faculty of Sciences of Nature and Life - Department of Biotechnology, Algeria , BENCHABANE, Messaoud University of Blida - Faculty of Sciences of Nature and Life - Department of Biotechnology, Algeria , TOUMI, Mohamed Superior Normal School - Department of Biology, Algeria , BELKACEM, Nesrine Faculty of Sciences of Tunis, University Campus - Department of Biology, Laboratory of Microorganisms and Active Biomolecules, Tunisia , GUESMI, Amel Faculty of Sciences of Tunis, University Campus - Department of Biology, Laboratory of Microorganisms and Active Biomolecules, Tunisia , AMEUR, Cherif University of Manouba - LR11-ES31 Biotechnology and Valorization of Bio-Geo Resources, Higher Institute for Biotechnology - Sidi Thabet Biotechpole, Tunisia , LECOMTE, Pascal l’Institut national de la recherche agronomique (INRA) - Institut des Siences de la Vigne et du Vin (ISVV) - Unité Mixte de Recherche (UMR) 1065 Plant Health and Agroecology Vineyard, France , MERAH, Othmane University of Toulouse - Institut National du Patrimoine-L École Nationale Supérieure des Ingénieurs en Arts Chimiques et Technologiques (INP-ENSIACET) - Agro-Industrial Chemistry Laboratory, France , MERAH, Othmane l’Institut national de la recherche agronomique (INRA) - Unité Mixte de Recherche (UMR) 1010 Contrat d Accueil et d Intégration (CAI), France
Abstract :
Grapevine dieback is an increasing problem in grape vineyards in Algeria. We conducted field surveys each spring between 2006 and 2012 to determine the fungal pathogens associated with grapevine decline in various grape-growing regions. We isolated and identified 3 species of Botryosphaeriaceae (Botryosphaeria dothidea, Diplodia seriata, and Neofusicoccum parvum) from infected wood using a combination of morphological and cultural characters, as well as phylogenetic analyses of ribosomal DNA internal transcribed spacer (ITS) regions and fruiting body. We identified 20 isolates of Diplodia seriata, 13 isolates of Botryosphaeria dothidea, and 7 isolates of Neofusicoccum parvum. Inoculation of grapevine plantlets with the 3 Botryosphaeriaceae species produced necrosis and vascular lesions in the wood after a 6-week incubation; Neofusicoccum parvum isolates were the most virulent, followed by D. seriata and B. dothidea. All 3 species were re-isolated from lesions on infected plantlets. Our results represent the first report of a canker disease of grapevine associated with species of the Botryosphaeriaceae in Algeria.
