Author/Authors :
Yang, Xiao-Kun General Hospital of Chengdu Military Command Area - Department of Emergency, China , Yang, Xiao-Kun Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Yang, Ya-Dong Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Tang, Shu-Qian Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Xu, Ling Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Yang, Gui-Hong Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Xu, Qiao-Yu Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Tang, Hui Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China , Wu, Jin-Jin Third Military Medical University - Daping Hospital, Research Institute of Wound Healing and Tissue Engineering of Chongqing - Department of Dermatology, China
Abstract :
Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism. Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and antimetastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and ECAD. Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p 0.05).Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.
Keywords :
Polysaccharide , Scutellaria barbata , 95 , D cell lines , Invasion , Metastasis
