Author/Authors :
Yang, Li Henan Normal University - School of Environment, China , Yang, Li Henan Key Laboratory for Environmental Pollution Control, China , Yang, Li Ministry of Education - Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, China , Yang, Li Henan Institute of Science and Technology - Department of Experimental Center, China , Yan, Qing-Hua Xinxiang Medical University - Department of Life Science and Technology, China , Ma, Jin-You Henan Institute of Science and Technology - Department of Experimental Center, China , Wang, Qing Henan Institute of Science and Technology - Department of Experimental Center, China , Zhang, Jian-Wei Henan Institute of Science and Technology - Department of Experimental Center, China , Xi, Guo-Xi Henan Normal University - School of Environment, China , Xi, Guo-Xi Ministry of Education - Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, China , Xi, Guo-Xi Henan Key Laboratory for Environmental Pollution Control, China
Abstract :
Purpose: To develop a simple, precise and rapid high-performance liquid chromatographic technique coupled with photodiode array detection (DAD) method for the simultaneous determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis. Methods: Ultrasound-assisted extraction was applied to isolate six antioxidant phenolic compounds from propolis samples. Chromatographic separation was performed using an Eclipse XDB-C18 column (150 mm×4.6 mm, 5 μm) by isocratic elution at 25°C. The mobile phase consisted of phosphate buffered saline (pH = 4.5) and methanol (40/60, v/v). The flow rate was set at 0.8 ml/min and the effluent was monitored using a DAD set at 260 nm. Results: Excellent linear correlation coefficient was observed in the range of 0.9977 to 0.9997. The reproducibility of the method was 1.58 % (RSD) and accuracy was confirmed with average recovery ranging from 95.71 to 104.26 %. Six antioxidant phenolic compounds in propolis samples were successfully determined within 50 min. Conclusion: The proposed isocratic LC method gave good separation and accurate determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis. The proposed method showed good sensitivity, linearity, repeatability and accuracy.
Keywords :
Propolis , Rutin , Quercetin , Luteolin , Genistein , Galangin , Curcumin , HPLC.
