Author/Authors :
Park, Won Sun Kangwon National University - School of Medicine - Department of Physiology, South Korea , Lee, Kyung Soo Inje University - College of Medicine - Department of Preventive Medicine, South Korea , Chun, Jin Ho Inje University - College of Medicine - Department of Preventive Medicine, South Korea , Urm, Sang Hwa Inje University - College of Medicine - Department of Preventive Medicine, South Korea , Lee, Dae-Sung Pohang University of Science and Technology (POSTECH) - Ocean Science and Technology Institute, South Korea , Lee, Da-Young Inje University - College of Medicine - Department of Microbiology, South Korea , Park, Sae-Gwang Inje University - College of Medicine - Department of Microbiology, South Korea , Seo, Su-Kil Inje University - College of Medicine - Department of Microbiology, South Korea , Heo, Soo-Jin Korea Ocean Research and Development Institute - Marine Living Resources Research Department, South Korea , Qian, Zhong-ji Pukyong National University - Center for Marine-Integrated Biomedical Technology (BK21 Plus) - Department of Biomedical Engineering, South Korea , Jung, Won-Kyo Pukyong National University - Center for Marine-Integrated Biomedical Technology (BK21 Plus) - Department of Biomedical Engineering, South Korea , Choi, Il-Whan Inje University - College of Medicine - Department of Microbiology, South Korea
Abstract :
Purpose: To determine whether an ethanol extract from Callophyllis japonica (C. japonica) could attenuate indices of airway inflammation in a murine model of ovalbumin (OVA)-induced asthma. Methods: The free radical scavenging activity of the C. japonica ethanol extracts (CJE) wereinvestigated using an electron spin resonance (ESR) system. To make develop animal model of asthma, mice were sensitized and challenged with OVA. Results: CJE exhibited considerable scavenging activity of 71.08 ± 0.73, 79.11 ± 6.04%, 75.95 ± 7.01%, and 48.56 ± 5.96% of DPPH, alkyl, superoxide, and hydroxyl radicals, respectively. The successive intraperitoneal administration of CJE reduced the number of eosinophils in bronchoalveolar lavage (BAL) fluid, development of airway hyperresponsiveness (AHR), an increase in pulmonary Th2 cytokines, and allergen-specific immunoglobulin E (IgE). Conclusion: Administration of CJE markedly alleviates all indices of airway inflammation. This study provides evidence that CJE plays a critical role in the amelioration of the pathogenetic process of allergic asthma in mice.
Keywords :
Asthma , Phenolic contents , Free radical scavenging , Airway hyper , responsiveness , Cytokines , Immunoglobulin E
