A comparative study on phytochemical potentials of Rubus loganobaccus L.

Raspberries composed of various varieties, are popular ingredients of daily diet with highly distinguished biological activities. In this study, a comparative investigation was conducted on various chemical potential of methanol extracts from Rubus loganobaccus L. leaf parts cultured in greenhouse and open-field. Biochemical activity of the extracts obtained from the field cultured leaves were observed to be higher than greenhouse cultured plants. In the antioxidant 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the field cultured leaves were better succeeded in radical scavenging with IC50 as low as 1.08 ± 0.75 μg/mL while in reducing power assay, open-field plants against greenhouse plants had a higher EC50 (2.82 ± 0.70 and 2.41 ± 0.75 μg/mL, respectively). Considerable antibacterial and antifungal activity were observed in open-field plants and greenhouse plants in minimum inhibitory concentrations (MIC) and minimum bacterial concentration (MBC) tests with a similar pattern, in which the lowest MIC and MBC in open-field and greenhouse plants were 5 mg/mL and 20 mg/mL, respectively, against Bacillus cereus and pseudomonas aeruginosa. This experiment revealed that leaf parts of Rubus loganobaccus cultured in greenhouse and in field possess a number of biological properties including antioxidative and antimicrobial potentials with the superiority of the later source which indicates the criticality of using natural products to improve human health.