Title of article :
Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties
Author/Authors :
YAP, Fei-Ling International Medical University - Faculty of Medicine, Malaysia , CHEONG, Soon-Keng Universiti Tunku Abdul Rahman - Faculty of Medicine Health Sciences, Malaysia , AMMU, Radhakrishnan International Medical University - Faculty of Medicine , LEONG, Chooi-Fun Universiti Kebangsaan Malaysia - Faculty of Medicine, Malaysia
Abstract :
In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immnophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.
Keywords :
Mesenchymal stem cells , transfection , differentiation , immunophenotyping
Journal title :
The Malaysian Journal of Pathology
Journal title :
The Malaysian Journal of Pathology
