Title of article :
Predictive Role of Dihydropyrimidine Dehydrogenase (DPD) and Thymidylate Synthase (TS) Gene Expressions in Patients with Metastatic Colorectal Cancer Treated with Capecitabine
Author/Authors :
ABD EL-KADER, MOHAMAD Beni Sweefl University - Departments of Clinical Oncology, Egypt , ALAM, MAGDA Mansoura University - Departments of Clinical Oncology, Egypt , MAZROH, MOHAMAD Zagazig University - Departments of Clinical Oncology, Egypt , EL-SHERBINY, MAMDOUH Beni Suef University - Departments of Clinical Oncology, Egypt , MOHAMED, NADER Beni Sweefl University - Faculties of Medicine - Departments of Clinical Oncology, Egypt , GHARIB, AMAL F. Zagazig University - Department of Biochemistry, Egypt
From page :
11
To page :
18
Abstract :
Background and Objective: Dihydropyrimidine dehydrogenase (DPD) and thymidylate synthase (TS) gene expressions in metastatic colorectal cancer have been reported to be predictive parameters for the efficacy of fluoropyrimidinebased chemotherapy. In present study, we investigated the association between both DPD and TS expressions in metastatic colorectal cancer and the response and survival in patients treated with capecitabine. Patients and Methods: DPD and TS expressions were measured by reverse transcription-PeR in tissues obtained by ultrasound-guided needle biopsy from 37 patients who went on to receive oral treatment of capecitabine for metastatic colorectal cancer. Relative mRNA amounts of DPD or TS were expressed as the ratios of targeted gene to glyceraldehyde-3-phosphate dehydrogenase reverse transcription-PeR products. Results: Median values of DPD mRNA expressions were 0.30 and 0.65 for responding tumors and nonresponding ones, respectively, with a statistical significance (p 0.0001). No responding tumor had a DPD mRNA expression ≥0.5. A total of 19 tumors had low DPD mRNA expressions of 0.5, and 63% of them showed response. There was no responding tumor with both high DPD and high TS (TS mRNA expression 1.0). However, the response rate was 75% in tumors with both low DPD and low TS. The median survival time was 16.3 months in patients with both low DPD and low TS versus 8.4 months in patients with high DPD or high TS mRNA expression. Conclusion: In conclusion, the combination of DPD and TS mRNA expressions in metastatic tumor might be useful as predictive parameters for the efficacy of fluoropyrimidinebased chemotherapy for metastatic colorectal cancer.
Keywords :
Colo rectal cancer , Dihydropyrimidine dehydrogenase , Thymidylate synthase , Predictive marker.
Journal title :
The Medical Journal of Cairo University
Journal title :
The Medical Journal of Cairo University
Record number :
2538716
Link To Document :
بازگشت