Relation of Factor V Leiden Mutation to Repeated Implantation Failure in Women Undergoing Intra Cytoplasmic Sperm Injection (ICSI)

Objective: To determine whether women with factor V Leiden mutation, a common inherited defect of coagulation, are at increased risk for recurrent pregnancy loss after ICSI.Patients and Methods: Women with recurrent implantation failure were compared to a matching group of women conceived naturally. Factor V Leiden was investigated in each group to determine its homozygosity or heterozygosity and its relation and effect on implantation and ICSI. Study of Factor V was done through its purification, then amplification and detection using real-time PCR (Polymerase Chain Reaction).Results: No statistical significant difference was found between FVL mutation in cases of repeated implantation failure and controls who achieved natural conception (OR: 0.190 in cases and 0.333 in controls). Also No association were found in Age, BMI, Prevelance of Hypertension, History of DVT between cases with repeated implantation failure and controls who achieved natural pregnancy.Conclusion: It seems there is no association between Factor V Leiden mutation gene and repeated implantation failure in women undergoing ICSI, and so routine anticoagulant use for these women is not recommended.