The Additive Beneficial Antioxidant Effect of Ozone Therapy to Xanthine Oxidase Inhibitor in Experimentally-Induced Hyperuricemic Rats

The high serum uric acid level is termed as hyperuricemia, hyperuricemia has been demonstrated to play a pivotal role in the pathogenesis of various cardiovascular disorders and renal diseases. Because ozone therapy can activate the antioxidant system, influencing the level of antioxidant enzymes and some markers of endothelial cell damage, medical ozone treatment may be of help in the treatment of hyperuricemia. The aim of this study was to demonstrate the additive effect of ozone therapy to xanthine inhibitor on hemodynamic parameters, renal dysfunction and oxidative stress markers in hyperuricemic rats. Rats were subdivided into 4 groups: 1- Control rats, 2- Hyperuricemic non-treated rats, 3- Hyper-uricemic allopurinol-treated rats and 4- Hyperuricemic allopurinol + ozone-treated rats. At the end of the experiment, all rats were fasted for 12 hours. Systolic blood pressure (SBP) was measured, retro-orbital blood samples were collected for estimation of erythrocytes superoxide dismutase (SOD) activity, plasma malondialdehyde (MAD), serum uric acid (UA), serum urea and creatinine, the animals were then scarified. Renal artery was exposed for direct estimation of renal blood flow velocity (BFV) and renal vascular resistance (RVR). Ozone therapy significantly lowered serum UA, the arterial blood pressure, renal vascular resistance, and the plasma (MAD), also elevated significantly the erythrocytes (SOD) activity by its anti oxidative effect and improved renal blood flow velocity in hyperurcemic rats.