Quantitative Analysis of Testicular Biopsies in Acquired Obstructive Azoospermic Patients

Objectives: To assess quantitative deterioration of the testicular spermatogenic capacity in Obstructive Azoospermia (OA) in relation to the duration of obstruction and the patients age Methods: Retrospective analysis of forty seven histopathology testicular biopsy slides of acquired obstructive azoospermic patients who underwent TESE/ICSI, were analyzed using Johnsen score and late spermatid score. Biopsies with sperm granulomas were recorded. Results: The mean patient age was 41.8 years with a mean obstructive duration of 12 years. Only five slides showed the presence of sperm granuloma. No statistical correlation was found between Johnsen score and interval of obstruction (p=0.574) nor the age of the patient (p=0.506). No correlation was found between late spermatid score and interval of obstruction (p=0.113) or the patient s age (p=0.401). Conclusion: Duration of obstruction, patient s age and presence of sperm granulomas do not affect spermatogenic capacity proved by 2 quantitative analyses for the first time.