Author/Authors :
FAYEZ, ASHRAF Cairo University - Faculty of Medicine - Department of Andrology, Egypt , AL-AHWANI, SARWAT Cairo University - Faculty of Medicine - Department of Obstetrics Gynecology, Egypt , EL-GUINDI, AHMED Cairo University - Faculty of Medicine - Department of Andrology, Egypt , ALAHWANI, AMR Cairo University - Faculty of Medicine - Department of Andrology, Egypt , ZAKI, SHADY Cairo University - Faculty of Medicine - Department of Andrology, Egypt , ABD EL-RAHMAN, ISLAM FATHY SOLIMAN Cairo University - Faculty of Medicine - Department of Andrology, Egypt
Abstract :
Introduction: standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Purpose: We aimed to study the effect of different thawing temperatures on the motility recovery of cryopreserved human sperm. Patients and Methods: Fifty semen samples of males complaining of primary infertility and oligoasthenoteratozoospeтrniа. A semen sample was obtained and evaluated. Every sample was divided into three straws that underwent cryopreservation, then thawing at three different temperatures; 25°C, 37°C and 40°. Pre-cryopreservation sperm motility was compared to the post-thawing motility at each of the three thawing temperatures.Results: Total motility percentage was better at thawing temperatures of 37°C and 40°C compared to 25°C. Rapidly progressive motility was the highest at thawing temperature 40°C compared to the other thawing temperatures; 25°C and 37°C as p-value was 0.08, 0.001 and 0.001 respectively denoting no significant difference in change of the rapidly progressive motility before cryopreservation and at thawing temperature 40°C. Conclusion: Thawing cryo-preserved semen samples with severe oligoasthenoteratozoospermia at 40°C provides highest potential for restoring sperm motility.
