Effect of Erythropoietin on Bone Marrow Derived Mesenchymal Stem Cells Growth and Proliferation

Background: Bone marrow mesenchymal stem cells (BМMSСs) can differentiate into osteob asts, adipocytes, and other mеsеnстуmal cell lineages. These cells can also be expanded in culture for long periods without any apparent loss of differentiation capacity. Erythropoietin (END), is a molecule produced by kidney, and stimulates the proliferation and differentiation of erythroid progenitor cells. EPO can stimulate the proliferation and protraction of endothelial progenitor cells, and plays an important role in the proliferation and differentiation of marrow-derived mesenchymal stem cells. Objectives: We aimed to investigate the possible protective effect of EPO, especially anti-apoptotic effect on MSCs growth. Methods: Isolated bone marrow-derived mesenchymal stem cells from rats were treated with 10 IU/mL EPO for 5 days the cells were examined for the following:- Gene expression of oaspasе-3, ВАХ ВСL-2 ratio and STAT-5. - Cell proliferation assay. Results: Treatment of MSCs with EPO for only 5 days promotes MSCs growth and proliferation. ЕРО significantly increase gene expression of STAT-5 and BCL2 while significantly decrease gene expression of BAX andcaspase-3 with decreasing ВАХ/ВСL2. ЕРО also increase cell proliferation assay. Conclusion: Through anti-apoptotic effect, ЕРО promote MSCs growth and proliferation.