Author/Authors :
Harraz, Ahmed M. University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Harraz, Ahmed M. Mansoura University - Urology and Nephrology Centre, Egypt , Lin, Guiting University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Banie, Lia University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Wang, Guifang University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Shindel, Alan W. University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Huang, Yun-Ching Chang Gung University - College of Medicine - Graduate Institute of Clinical Medical Sciences, Taiwan , Huang, Yun-Ching University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Fandel, Thomas M. University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Garcia, Maurice University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Lue, Tom F. University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA , Lin, Ching-Shwun University of California - Knuppe Molecular Urology Laboratory - Department of Urology, USA
Abstract :
Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function. Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining. Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. How ever, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells. Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.
Keywords :
Derived stem cells , Ileocystoplasty , Conscious cystometry , 5 , ethynyl , 2 , deoxyuridine
