Title of article :
Clinicopathologic Study of a Series of Giant Cell Fibroma Using Picrosirius Red Polarizing Microscopy Technique
Author/Authors :
Datar, Uma Vasant MGV’s KBH Dental College - Department of Oral Pathology and Microbiology, India , Mohan, Bhavna Chulliparampil KLE University - KLE VK Institute of Dental Sciences - Department of Oral Pathology and Microbiology, India , Hallikerimath, Seema KLE University - KLE VK Institute of Dental Sciences - Department of Oral Pathology and Microbiology, India , Angadi, Punnya KLE University - KLE VK Institute of Dental Sciences - Department of Oral Pathology and Microbiology, India , Kale, Alka KLE University - KLE VK Institute of Dental Sciences - Department of Oral Pathology and Microbiology, India , Mane, Deepa KLE University - KLE VK Institute of Dental Sciences - Department of Oral Pathology and Microbiology, India
From page :
746
To page :
749
Abstract :
Background: Giant cell fibroma (GCF) is a distinct type of fibroma with characteristic large, stellate mononuclear or multinucleated giant fibroblasts; the stroma ofGCF is relatively unexplored. The Picrosirus red polarizing microscopy technique is used to characterize the collagen fibers. The aim of this study was to evaluate the staining properties of collagen fibers in GCF and to correlate it with fibroma using Picrosirius red under the polarizing microscope and van Gieson under bright field microscope. Methods: In the present study van Gieson and picrosirius red stained slides of 7 cases each of GCF and fibroma were compared for the staining properties of collagen. Using picrosirus red polarizing microscopy; colors noted in fibroma included yellow, yellowish-orange and green, whereas the GCF showed predominantly yellow and orange colors. In Van Gieson stained sections it was observed that the collagen in GCF was densely packed and arranged perpendicular to the epithelium while the collagen in fibroma was loosely packed and arranged parallel to the epithelium. Conclusion: Observable differences in the stroma of the collagen of GCF and fibroma were noted. Collagen in GCF was more mature and dense. The Picrosirius red polarizing technique can be used to assess the collagen in GCF.
Keywords :
GCF , Picrosirius red , polarizing microscopy
Journal title :
Archives of Iranian Medicine
Journal title :
Archives of Iranian Medicine
Record number :
2545437
Link To Document :
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