Title of article :
Effect of Homocysteine and Protective Role of Trimethylglycine on the Structure of Tibia in Growing Albino Rats: Histological and Biochemical Study
Author/Authors :
Ragaie, Alyaa Future University - Faculty of Oral and Dental Medicine, Egypt , Ahmed, Mona Ain Shams University - Faculty of Girls - Zoology Department, Egypt , Kamal, Naglaa Ain Shams University - Faculty of Girls - Zoology Department, Egypt
From page :
198
To page :
207
Abstract :
Introduction: Homocysteine (HCY) is an amino acid that is formed as an intermediate during the metabolism of methionine. Epidemiological studies have shown that too much homocysteine in blood plasma leads to a higher risk of coronary heart disease and strokes. It is also characteristic for homocystinuria. Trimethylglycine TMG (known as betaine) is a methyl donor that helps the quick conversion of homocysteine into methionine. Aim of the Work: Is to investigate the structural and the biochemical changes that occur in the articular cartilage, the growth plate and the bone structure of tibia in growing albino rats after being treated with oral supplement of HCY and possible protective role of TMG. Material and Methods: Thirty two young male albino rats were used in this experiment. They were equally divided into four group. The control group received a balanced standard diet, HCY treated group received 0.6mgHCY/kg b.w day, TMG treated group (0.6gm TMG/kg b.wday) and an interacting group received both HCY and TMG. Rats were sacrificed every 2weeks up till the 8th week specimens from the tibias were obtained and processed for histological and biochemical study. Results: Indicated that examination of rat bones of HCY group showed apparently abnormal radial and longitudinal bone growth. H E stained sections of proximal ends of tibias showed changes in chondrocytes of proximal tibia. The growth plate of the proximal tibia showed a lack of the orderly chondrocytes columns arrangement. Examination of shafts of tibial bone of the same group showed many degenerative changes in periostum.The matrix contained many resorption cavities that were filled with granulation tissue. In addition there was evidence of abnormal biochemical patterns represented by low bone mineral density and bone mineral content. Statistically significant decrease in bone width compared to an increase in length and cross section area was detected. On the other hand TMG showed improved bone formation and inhibition of bone resorption. Conclusion: Was concluded that HCY produced profound histological changes in the articular cartilage, growth plate and bone structure of tibia of growing male albino rats.Mean while treatment with TMG markedly decreased the induced damage by HCY.
Keywords :
Homocysteine , trimethlglycine , tibial bone.
Journal title :
The Egyptian Journal of Histology
Journal title :
The Egyptian Journal of Histology
Record number :
2548001
Link To Document :
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