The effect of TGF-alpha on neurogenesis in sub ventricular zone of rat brain after ischemia-reperfusion

Introduction: Stroke is the third important reason of death in adults and an important cause of adult disability. Previous studies suggest that TGF-alpha can induce neurogenesis after stroke. Here in, we studied neurogenesis effects of the TGF-alpha on subventricular zone following ischemia-reperfusion. Methods: Male wistar rats (250-300 g) were divided into ischemia and treatment groups. After inductreion of ischemia-reperfusions, PBS (phosphate buffer salin) and TGF-alpha 50 ng were injected stereotaxicaly in lateral ventricle in ischemia and treatment respectively. After 12 days, the nestin expression in subventricular zone was assessed by immunohistochemical staining method. Results: Our results showed that nestin expression increased significantly in treatment group in comparison with ischemia group (p 0.05). Discussion: Expression of nestin in SVZ indicates that TGF- a can stimulate the neural stem cells proliferation after ischemia - reperfusion injury.