Immunoexpression of gelatinase and apolipoprotein E ininduced glomerulosclerosis in adult male albino rat

IntroductionThe abnormal expressions of gelatinase are implicated in the pathogenesis ofextracellular matrix accumulation in glomerulosclerosis (GS). ApolipoproteinE (apoE) is an important plasma protein in cholesterol that plays a key role in theprogression of GS.AimThe aim of this work was to study the immunoexpression of gelatinases and apoEin experimentally induced GS.Materials and methodsTwenty male rats were divided into two equal groups: a control group and a GSmodel group (each n=10). The GS was induced by an injection of adriamycin(5 mg/kg). At the end of 4 weeks, the 10 rats in each group were killed and kidneyspecimens were processed for (histological and immunohistochemical study)biochemical studies.ResultsSerum total protein and serum albumin in the GS group were reduced comparedwith those of the control group (P 0.01). Compared with the control group, thevalues of 24-h urine total protein, 24-h urine excretion for albumin, blood ureanitrogen, serum creatinine, and GS index in the GS group were significantlyincreased (P 0.01). In the GS group, there was glomerular hypercellularity andhypertrophy with focal obliteration of some capillaries. Interstitial fibrosis andinflammation were detected. The immunostaining for gelatinase was decreased,whereas apoE, transforming growth factor-β1, and α-smooth muscle actin wereincreased.ConclusionIn induced GS, an increased expression of apoE was associated with decreasedexpression of gelatinase and this led to accumulation of extracellular material inglomeruli.