The relationship between hormones level and body mass index with insertion and deletion (D/I) polymorphism of ACE gene in infertile patients with polycystic ovary syndrome

Background: The prevalence of polycystic ovary syndrome (PCOS) and infertility are rising due to changes in lifestyle. In this study, the polymorphism of insertion and deletion (I/D)in the angiotensin converting enzyme (ACE) gene in PCOS patients and the possibility of its association with PCOS and plasma level of hormones level as well as body mass index was investigated. Materials and Methods: In this study, three polymorphisms in the ACE gene in Iranian women (Insertion-Insertion (II), Insertion-Deletion (ID), Deletion-Deletion (DD)) at three groups: PCOS, infertile patients and control as well as their relationship with hormones level and Body Mass Index (BMI) were examined. The methods included standard DNA extraction from peripheral blood and polymerase chain reaction (PCR) using specific primers for ACE gene. The data were then statistically analyzed. Results: Results showed an higher prevalence of ID polymorphism in PCOS and infertility population with statistically significant association (p=0.013). There were also significant associations between the polymorphism with BMI, LH and progesterone level. The number of primordial follicles also found to be three times greater in PCOS group compared with normal ones. Conclusion: The results showed significant association between polymorphisms of ACE gene, hormones level and BMI in PCOS which might be considered as possible prognostic marker for infertility of PCOS patients.