Wudani leaf extract (Quisqualis indica linn) as traditional medicine to control the incidence of cattle worm

Background: The present research work was aimed to study whether application of 10% extract of wudani leaf (Quisqualis indica Linn) may decrease the potential of becoming embryos of eggs of Fasciola gigantica and Paramphistomum sp. worms under in-vitro evaluation. Methods: It was used three doses of the extract; those were 0.5 ml/40 ml of physiological NaCl, 1.0 ml/40 ml NaCl and 2.0 ml/40 ml of NaCl and with 5 repetitions for each dose. Assessments of the ovicidal effect of extract that is reducing the potential of eggs to become embryo were conducted at day-10 and day-30 at the Parasitology Laboratory of The Faculty of Veterinary Medicine, Udayana University, Bali. Results: Results showed that assessment at day-10, the ovicidal ability of the extract at 0.5 ml/40 ml NaCl for Fasciola gigantica eggs was 23.8% and was significantly lower (P 0.05) than that of the dose of 1 ml/40 ml (33.1%) and of the dose of 2 ml/40 ml (35.9%). However, it was significantly higher than the control (10.4%). Moreover, the ovicidal ability of dose of 1 ml/40 ml and 2 ml/40 ml did not differ significantly (P 0.05). At Day-30, the ovicidal ability of 1 ml/40 and 2 ml/40 ml was very high, that was 88.8% and 88.5%, respectively. Furthermore, for eggs of Paramphistomum sp., assessment at day-10 showed that the ovicidal ability of the dose of 2 ml/40 ml was the highest (23.3%) and significantly higher (P 0.05) than that of the dose of 1 ml/40 ml (21.6%), 0.5 ml/40 ml (11.6%) and of the control (9.4%). On the other hand, there were no significant difference were noted between the dose of 1 ml/40 ml, 2 ml/ 40 ml and the control (P 0.05). Likewise, at day-30 of assessment, the ovicidal ability of 1 ml/40 ml (51.9%) and 2 ml/40 ml (53.4%) was significantly higher (P 0.01) than that of control. The high ovicidal ability of 10% extract of wudani leaf at the dose of 1 – 2 ml/40 ml NaCl may be related to damage of egg shells caused by active ingredient of the extract. Conclusion: Since infection of lungworm predominantly occurs in cattle, the present results may reveal the opportunity of applying the wudani leaf extract to control lungworm infection in cattle. Further study is definitely needed.