Screening drugs-potential immunomodulators for T-2 mycotoxicosis

The aim of the research was to study the effectiveness of substances with an immunostimulating effect in T-2 mycotoxicosis. Subacute T-2 mycotoxicosis was simulated in male, white Wistar rats by administering intragastrically, a toxic at a dose of 1/5 LD50 (0.64 mg/kg of body weight) for 15 days, at the same time the animals were immunized with the vaccine against colibacteriosis (on the first day of the experiment). The following drugs were tested: “Xymedon” (1-(β-oxyethyl)-4,6-dimethyl-1,2-dihydro-2-oxopyrimidine) was administered intragastrically at a dose of 75 mg/kg daily; “Dimephosphone” (Dimethyloxobuthylphosphonilmethylate) at a dose of 90 mg/kg daily; “Levamisole” (S)-2,3,5,6-Tetrahydro-5-phenylimidazo[2,1-b]thiazole hydrochloride) intragastrically at a dose of 4 mg/kg for the first 3 days, after a 4-day break it was administered again for 3 days; “Thymalin” (thymus extract derived from thymus glands of large animals) administered intramuscularly at a dose of 0.2 mg/kg for 5 days from 7th day of the experiment. All experimental animals were immunized with the vaccine against colibacteriosis; the vaccine was administered intramuscularly into the back of the thigh at a dose of 0.5 ml per day. The criteria for evaluating the effectiveness of the drugs were hematological indicators, immunological indicators and accumulation of specific antibodies to the vaccine. It has been established that all the tested drugs had a protective effect which was expressed in positive changes in hematological, immunological and non-specific resistance indicators. “Thymalin” had the most pronounced protective effect in toxicosis with T-2 toxin in rats, “Xymedon” had the least protective effect, “Dimephosphone” had an average level of effect. “Thymalin” proved to be more effective according to the indicators of the accumulation of specific antibodies.