Author/Authors :
Karami, Ali baqiyatallah university of medical sciences - Research Center of Molecular Biology, Institute of Military Medicine - Department of Molecular Medicine, تهران, ايران , Karami, Ali National Institute for Genetic Engineering and Biotechnology (NIGEB) - Department of Immunology, ايران , Sarbolouki, Mohammad Nabi university of tehran - Institute of Biophysics and Biochemistry, تهران, ايران , Khatami, Fatemeh National Institute for Genetic Engineering and Biotechnology (NIGEB) - Department of Immunology, ايران , Khatami, Fatemeh imam hossein university - Faculty of Basic Science - Department of Biology, تهران, ايران , Rastgoo, Nasrin National Institute of Genetic Engineering and Biotechnology - Department of Molecular Genetic, ايران
Abstract :
The immunogenicity and protective efficacy of DNA vaccines have been demonstrated in numerous animal models of infectious diseases. In order to increase the potency of DNA vaccines, in this study, conventional adjuvants such as aluminium phosphates, dendrosome, CpG motif and mixture of aluminium phosphate and CpG motif have been tested. Female BALB/c mice were immunized with mixture of 10, 25 and 50 μg HCV core pcDNA3. Each dose of recombinant pcDNA3 together with different adjuvants used as an immunogen were injected three times on day; 0, 30 and 50 days. Blood samples were collected at four different times intervals and antibody response against HCV core antigen was determined by HCV core ELISA kit. The results indicate that the best antibody response was with mixture of aluminium phosphate and CpG motif as an adjuvant. This data suggest that the antibody response induced following DNA immunization can be modified by formulation strategies.
Keywords :
CpG Motif , Dendrosome, Hepatitis C Virus , HCV Core pcDNA3
