Author/Authors :
Nassiry, Mohammad Reza ferdowsi university of mashhad, مشهد, ايران , Norouzy, Amir ferdowsi university of mashhad, مشهد, ايران , Eftekhari Shahroudi, Fereidoun ferdowsi university of mashhad, مشهد, ايران , Javadmanesh, Ali ferdowsi university of mashhad, مشهد, ايران , Shad, Mohammad Ali Jahad-Keshavarzi Khorasan
Abstract :
To prevent distribution of recessive alleles in dairy herds all bulls used for Al (Artificial Insemination) have to be tested. In this study 26 blood and 4 semen samples were supplied from Iranian Holstein bulls used for Al. Genomic DNA was extracted from 100 \xl of blood and 200 \xl of semen. Samples were tested by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. PCR reaction was performed for amplification of polymorphic region of the CD 18 gene on chromosome 1 and exon 5 of ASS gene on chromosome 11. We detected one Bovine Leukocyte Adhesion Deficiency (BLAD) carrier and no carrier for bovine Citrolliemia in this study. Hardy-Weinberg test confirmed the equilibrium of BLAD locus in this population.
Keywords :
BLAD , CD 18 , Citrullinemia , Argininosuc , cinate Synthetase , Holstein , PCR , RFLP
